Review



total creb (tcreb  (Upstate Biotechnology Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Upstate Biotechnology Inc total creb (tcreb
    Total Creb (Tcreb, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+creb+(tcreb/total+creb++tcreb+antibody/10__1074_slash_jbc__m010450200-65-15-28
    Average 90 stars, based on 1 article reviews
    total creb (tcreb - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: β-Amyloid-(1–42) Impairs Activity-dependent cAMP-response Element-binding Protein Signaling in Neurons at Concentrations in Which Cell Survival Is Not Compromised
    Article Snippet: Following incubation with the primary antibodies specific for either Ser-133 phosphorylated CREB (P-CREB) (1:2,000) or total CREB (TCREB), which recognize both phospho- and dephospho-CREB (1:1,000) (both antibodies from Upstate Biotechnology, Inc.), the blots were washed in PBS containing 0.1% Tween and then incubated with the secondary antibody, goat anti-rabbit IgG conjugated with horseradish peroxidase (Vector Laboratories), at 1:8000 dilution in the blocking solution for 60 min. Blots were then washed four times with PBS containing 0.1% Tween.

    Blocking Assay:

    Article Title: β-Amyloid-(1–42) Impairs Activity-dependent cAMP-response Element-binding Protein Signaling in Neurons at Concentrations in Which Cell Survival Is Not Compromised
    Article Snippet: Following incubation with the primary antibodies specific for either Ser-133 phosphorylated CREB (P-CREB) (1:2,000) or total CREB (TCREB), which recognize both phospho- and dephospho-CREB (1:1,000) (both antibodies from Upstate Biotechnology, Inc.), the blots were washed in PBS containing 0.1% Tween and then incubated with the secondary antibody, goat anti-rabbit IgG conjugated with horseradish peroxidase (Vector Laboratories), at 1:8000 dilution in the blocking solution for 60 min. Blots were then washed four times with PBS containing 0.1% Tween.



    Similar Products

    alphalisa surefire ultratm creb total assay kit  (Revvity)
    90
    Revvity alphalisa surefire ultratm creb total assay kit
    a Pictures of Ctsd +/+ and Ctsd − /− PyMT cells cultured for 7–10 days in 10% FCS or 1% FCS medium. Bars, 50 μm. Representative of 5 independent experiments. b Staining for acidic β-galactosidase in 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells. Bars, 50 μm. Representative of 3 independent experiments. c Quantification of label-retaining cells among 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells ( n = 7 independent experiments; two-sided two-sample t -test). d Amount of Annexin-V - 7-AAD − (living) cells among 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells treated with Navitoclax, relative to DMSO control and Ctsd +/+ ( n = 3 independent experiments; two-sided one-sample t -test). e Quantification of IL-6 by <t>alphaLISA</t> in cell-conditioned media from 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells ( n = 2 independent experiments). f Relative Cdkn2a/p16 and Cdkn1a/p21 expression determined by RT-PCR in 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells ( n = 3 independent experiments; two-sided two-sample t -test). Bar charts show all data points with mean + SD and p -value. Source data are provided as a Source Data file.
    Alphalisa Surefire Ultratm Creb Total Assay Kit, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+creb+(tcreb/AlphaLISA+SureFire+Ultra+total+CREB+Assay+Kit%2C+100+Assay+Points/pmc07552405-457-15-42
    Average 90 stars, based on 1 article reviews
    alphalisa surefire ultratm creb total assay kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    alphalisa surefire p creb assay kit  (Revvity)
    90
    Revvity alphalisa surefire p creb assay kit
    a Pictures of Ctsd +/+ and Ctsd − /− PyMT cells cultured for 7–10 days in 10% FCS or 1% FCS medium. Bars, 50 μm. Representative of 5 independent experiments. b Staining for acidic β-galactosidase in 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells. Bars, 50 μm. Representative of 3 independent experiments. c Quantification of label-retaining cells among 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells ( n = 7 independent experiments; two-sided two-sample t -test). d Amount of Annexin-V - 7-AAD − (living) cells among 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells treated with Navitoclax, relative to DMSO control and Ctsd +/+ ( n = 3 independent experiments; two-sided one-sample t -test). e Quantification of IL-6 by <t>alphaLISA</t> in cell-conditioned media from 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells ( n = 2 independent experiments). f Relative Cdkn2a/p16 and Cdkn1a/p21 expression determined by RT-PCR in 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells ( n = 3 independent experiments; two-sided two-sample t -test). Bar charts show all data points with mean + SD and p -value. Source data are provided as a Source Data file.
    Alphalisa Surefire P Creb Assay Kit, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+creb+(tcreb/AlphaLISA+SureFire+Ultra+total+CREB+Assay+Kit%2C+100+Assay+Points/pm30656694-76-8-13
    Average 90 stars, based on 1 article reviews
    alphalisa surefire p creb assay kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    total creb tcreb 48h2 antibodies  (Cell Signaling Technology Inc)
    94
    Cell Signaling Technology Inc total creb tcreb 48h2 antibodies
    Effect of rolipram on phosphorylated <t>CREB</t> (pCREB) and total <t>(tCREB)</t> expression in the testis after pelvic irradiation (IR) (2 Gy). (A) Western blot analysis of pCREB and tCREB expression after 12 hours and 35 days following pelvic irrdiation (2 Gy). Values are reported as the mean±standard deviation of five mice in each group (*p<0.05 as compared with the irradiation control group). (B) Representative images (×100) showing pCREB-stained testis sections from the sham control, rolipram+sham control, irradiation control, and rolipram+irradiation groups 35 days after irradiation. OR: odds ratio.
    Total Creb Tcreb 48h2 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+creb+(tcreb/CREB+Rabbit+mAb/pmc04412004-56-12-21
    Average 94 stars, based on 1 article reviews
    total creb tcreb 48h2 antibodies - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    tcreb (p) total creb antibody  (Cell Signaling Technology Inc)
    90
    Cell Signaling Technology Inc tcreb (p) total creb antibody
    Effect of rolipram on phosphorylated <t>CREB</t> (pCREB) and total <t>(tCREB)</t> expression in the testis after pelvic irradiation (IR) (2 Gy). (A) Western blot analysis of pCREB and tCREB expression after 12 hours and 35 days following pelvic irrdiation (2 Gy). Values are reported as the mean±standard deviation of five mice in each group (*p<0.05 as compared with the irradiation control group). (B) Representative images (×100) showing pCREB-stained testis sections from the sham control, rolipram+sham control, irradiation control, and rolipram+irradiation groups 35 days after irradiation. OR: odds ratio.
    Tcreb (P) Total Creb Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+creb+(tcreb/tcreb++p++total+creb+antibody/10__3233_slash_jad___121837-48-243-245
    Average 90 stars, based on 1 article reviews
    tcreb (p) total creb antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    rabbit anti-total creb (tcreb) antibody  (Cell Signaling Technology Inc)
    90
    Cell Signaling Technology Inc rabbit anti-total creb (tcreb) antibody
    Effect of rolipram on phosphorylated <t>CREB</t> (pCREB) and total <t>(tCREB)</t> expression in the testis after pelvic irradiation (IR) (2 Gy). (A) Western blot analysis of pCREB and tCREB expression after 12 hours and 35 days following pelvic irrdiation (2 Gy). Values are reported as the mean±standard deviation of five mice in each group (*p<0.05 as compared with the irradiation control group). (B) Representative images (×100) showing pCREB-stained testis sections from the sham control, rolipram+sham control, irradiation control, and rolipram+irradiation groups 35 days after irradiation. OR: odds ratio.
    Rabbit Anti Total Creb (Tcreb) Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+creb+(tcreb/p+creb+antibody/pm21337371-60-12-18
    Average 90 stars, based on 1 article reviews
    rabbit anti-total creb (tcreb) antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    antibody that specifically recognizes total (phosphorylated and unphosphorylated creb; tcreb)  (Millipore)
    90
    Millipore antibody that specifically recognizes total (phosphorylated and unphosphorylated creb; tcreb)
    Effect of rolipram on phosphorylated <t>CREB</t> (pCREB) and total <t>(tCREB)</t> expression in the testis after pelvic irradiation (IR) (2 Gy). (A) Western blot analysis of pCREB and tCREB expression after 12 hours and 35 days following pelvic irrdiation (2 Gy). Values are reported as the mean±standard deviation of five mice in each group (*p<0.05 as compared with the irradiation control group). (B) Representative images (×100) showing pCREB-stained testis sections from the sham control, rolipram+sham control, irradiation control, and rolipram+irradiation groups 35 days after irradiation. OR: odds ratio.
    Antibody That Specifically Recognizes Total (Phosphorylated And Unphosphorylated Creb; Tcreb), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+creb+(tcreb/antibody+that+specifically+recognizes+total++phosphorylated+and+unphosphorylated+creb++tcreb+/pm19248836-30-20-22
    Average 90 stars, based on 1 article reviews
    antibody that specifically recognizes total (phosphorylated and unphosphorylated creb; tcreb) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    total creb (tcreb  (Upstate Biotechnology Inc)
    90
    Upstate Biotechnology Inc total creb (tcreb
    Effect of rolipram on phosphorylated <t>CREB</t> (pCREB) and total <t>(tCREB)</t> expression in the testis after pelvic irradiation (IR) (2 Gy). (A) Western blot analysis of pCREB and tCREB expression after 12 hours and 35 days following pelvic irrdiation (2 Gy). Values are reported as the mean±standard deviation of five mice in each group (*p<0.05 as compared with the irradiation control group). (B) Representative images (×100) showing pCREB-stained testis sections from the sham control, rolipram+sham control, irradiation control, and rolipram+irradiation groups 35 days after irradiation. OR: odds ratio.
    Total Creb (Tcreb, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+creb+(tcreb/total+creb++tcreb+antibody/10__1074_slash_jbc__m010450200-65-15-28
    Average 90 stars, based on 1 article reviews
    total creb (tcreb - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    a Pictures of Ctsd +/+ and Ctsd − /− PyMT cells cultured for 7–10 days in 10% FCS or 1% FCS medium. Bars, 50 μm. Representative of 5 independent experiments. b Staining for acidic β-galactosidase in 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells. Bars, 50 μm. Representative of 3 independent experiments. c Quantification of label-retaining cells among 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells ( n = 7 independent experiments; two-sided two-sample t -test). d Amount of Annexin-V - 7-AAD − (living) cells among 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells treated with Navitoclax, relative to DMSO control and Ctsd +/+ ( n = 3 independent experiments; two-sided one-sample t -test). e Quantification of IL-6 by alphaLISA in cell-conditioned media from 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells ( n = 2 independent experiments). f Relative Cdkn2a/p16 and Cdkn1a/p21 expression determined by RT-PCR in 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells ( n = 3 independent experiments; two-sided two-sample t -test). Bar charts show all data points with mean + SD and p -value. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Cathepsin D deficiency in mammary epithelium transiently stalls breast cancer by interference with mTORC1 signaling

    doi: 10.1038/s41467-020-18935-2

    Figure Lengend Snippet: a Pictures of Ctsd +/+ and Ctsd − /− PyMT cells cultured for 7–10 days in 10% FCS or 1% FCS medium. Bars, 50 μm. Representative of 5 independent experiments. b Staining for acidic β-galactosidase in 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells. Bars, 50 μm. Representative of 3 independent experiments. c Quantification of label-retaining cells among 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells ( n = 7 independent experiments; two-sided two-sample t -test). d Amount of Annexin-V - 7-AAD − (living) cells among 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells treated with Navitoclax, relative to DMSO control and Ctsd +/+ ( n = 3 independent experiments; two-sided one-sample t -test). e Quantification of IL-6 by alphaLISA in cell-conditioned media from 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells ( n = 2 independent experiments). f Relative Cdkn2a/p16 and Cdkn1a/p21 expression determined by RT-PCR in 10% FCS and 1% FCS Ctsd +/+ and Ctsd − /− PyMT cells ( n = 3 independent experiments; two-sided two-sample t -test). Bar charts show all data points with mean + SD and p -value. Source data are provided as a Source Data file.

    Article Snippet: CREB, phosphorylated CREB and IL-6 levels in cells and cell-conditioned media were measured with the AlphaLISA® SureFire® UltraTM CREB Total Assay Kit (ALSU-TCREB-A500), AlphaLISA® SureFire® UltraTM p-CREB (Ser133) Assay Kit (ALSU-PCREB-A500), and the AlphaLISA Mouse Interleukin 6 (mIL6) Kit (AL504 C) from PerkinElmer, respectively.

    Techniques: Cell Culture, Staining, Control, Expressing, Reverse Transcription Polymerase Chain Reaction

    a Phospho-kinase antibody arrays with lysates from Ctsd +/+ and Ctsd − /− PyMT cells cultured for 7–10 days (1% FCS) or for ≥8 weeks (1% FCS LT) in 1% FCS medium. Changes in phosphorylation are plotted as log2 fold change (FC) for indicated comparisons ( n = 2 independent experiments). b , c Transcriptome analysis of 1% FCS and 1% FCS LT Ctsd +/+ and Ctsd − /− PyMT cells ( n = 3 independent experiments). b Change in expression of significantly regulated genes of the CREB gene set for indicated comparisons. Red dashed lines, borders for gene up- and downregulation (|log2 FC | > 1). c Clustering analysis of the log2 FC of CREB genes with significant upregulation in Ctsd − /− 1% FCS LT versus 1% FCS PyMT cells for indicated comparisons shown as heatmap. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Cathepsin D deficiency in mammary epithelium transiently stalls breast cancer by interference with mTORC1 signaling

    doi: 10.1038/s41467-020-18935-2

    Figure Lengend Snippet: a Phospho-kinase antibody arrays with lysates from Ctsd +/+ and Ctsd − /− PyMT cells cultured for 7–10 days (1% FCS) or for ≥8 weeks (1% FCS LT) in 1% FCS medium. Changes in phosphorylation are plotted as log2 fold change (FC) for indicated comparisons ( n = 2 independent experiments). b , c Transcriptome analysis of 1% FCS and 1% FCS LT Ctsd +/+ and Ctsd − /− PyMT cells ( n = 3 independent experiments). b Change in expression of significantly regulated genes of the CREB gene set for indicated comparisons. Red dashed lines, borders for gene up- and downregulation (|log2 FC | > 1). c Clustering analysis of the log2 FC of CREB genes with significant upregulation in Ctsd − /− 1% FCS LT versus 1% FCS PyMT cells for indicated comparisons shown as heatmap. Source data are provided as a Source Data file.

    Article Snippet: CREB, phosphorylated CREB and IL-6 levels in cells and cell-conditioned media were measured with the AlphaLISA® SureFire® UltraTM CREB Total Assay Kit (ALSU-TCREB-A500), AlphaLISA® SureFire® UltraTM p-CREB (Ser133) Assay Kit (ALSU-PCREB-A500), and the AlphaLISA Mouse Interleukin 6 (mIL6) Kit (AL504 C) from PerkinElmer, respectively.

    Techniques: Cell Culture, Phospho-proteomics, Expressing

    Effect of rolipram on phosphorylated CREB (pCREB) and total (tCREB) expression in the testis after pelvic irradiation (IR) (2 Gy). (A) Western blot analysis of pCREB and tCREB expression after 12 hours and 35 days following pelvic irrdiation (2 Gy). Values are reported as the mean±standard deviation of five mice in each group (*p<0.05 as compared with the irradiation control group). (B) Representative images (×100) showing pCREB-stained testis sections from the sham control, rolipram+sham control, irradiation control, and rolipram+irradiation groups 35 days after irradiation. OR: odds ratio.

    Journal: The World Journal of Men's Health

    Article Title: Protective Effect of Administered Rolipram against Radiation-Induced Testicular Injury in Mice

    doi: 10.5534/wjmh.2015.33.1.20

    Figure Lengend Snippet: Effect of rolipram on phosphorylated CREB (pCREB) and total (tCREB) expression in the testis after pelvic irradiation (IR) (2 Gy). (A) Western blot analysis of pCREB and tCREB expression after 12 hours and 35 days following pelvic irrdiation (2 Gy). Values are reported as the mean±standard deviation of five mice in each group (*p<0.05 as compared with the irradiation control group). (B) Representative images (×100) showing pCREB-stained testis sections from the sham control, rolipram+sham control, irradiation control, and rolipram+irradiation groups 35 days after irradiation. OR: odds ratio.

    Article Snippet: The membranes were then incubated with rabbit monoclonal pCREB (Ser133; 87G3) or total CREB (tCREB) (48H2) antibodies (both at 1:1,000 dilution; Cell Signaling Technology) in PBS-T overnight at 4℃.

    Techniques: Expressing, Irradiation, Western Blot, Standard Deviation, Staining